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Reversion by ozone treatment of acute nephrotoxicity induced by cisplatin in rats

Reversion by ozone treatment of acute nephrotoxicity induced by cisplatin in rats

Ricardo Gonza´ lez1, Aluet Borrego1,CA,
Zullyt Zamora1, Cheyla Romay1, Frank Hernández1,
Silvia Mene´ndez1, Teresita Montero2 and
Enis Rojas1

1Ozone Research Center, PO Box 6414, Cuba; 2‘Dr Luis
Díaz Soto’ Hospital, Cuba

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Research Communication

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Ozone therapy has become a useful treatment for pathological processes, in which the damage mediated by reactive oxygen species is involved. Several lines of evidence suggest that cisplatin-induced acute nephrotoxicity is partially mediated by reactive oxygen species.


To analyze the effect of ozone administration after cisplatin-induced acute nephrotoxicity.


Male Sprague-Dawley rats were treated with five intra-rectal applications of ozoneuoxygen mixture at 0.36, 1.1 and 1.8 mgukg after cisplatin intraperitoneal injection (6 mgukg). Serum and kidneys were taken off 5 days after cisplatin treatment. Creatinine was measured in the serum and the activities of antioxidant enzymes and thiobarbituric acid reactive substances and glutathione content were analyzed in renal homogenate.


Ozone treatment diminished the increase in serum creatinine levels, the glutathione depletion and also reversed the inhibition of superoxide dismutase, catalase and glutathione peroxidase activities induced by cisplatin in the rat kidney. Also, the renal content of thiobarbituric reactive substances was decreased by ozoneuoxygen mixture applied after cisplatin.


Intrarectal applications of ozone reversed the renal pro-oxidant unbalance induced by cisplatin treatment by the way of stimulation to some constituents of antioxidant system in the kidney, and thereby it decreased the renal damage.

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