Oxidative preconditioning affords protection against carbon tetrachloride induced Glycogen Depletion and Oxidative Stress in Rat
E. Candelario-Jalil,1 * S. Mohamed –Al-Dalain,1 O.S León Fernández,1 S. Menéndez,2 G. Pérez-Davison,1 N. Merino,3 S. Sam1 and H.H. Ajamieh1
1 Center for Research and Biological Evaluation, University of Havana, Institute of Pharmacy and Food Sciences, Apertado Postal 6079, Havana City 10600, Cuba
2 Ozone Research Center, Cuba
3 National Center for Scientific Research, Cuba.
Revista o sitio electrónico (URL) donde se publicó
JOURNAL OF APPLIED TOXICOLOGY
Ozone; oxidative preconditioning; carbon tetrachloride hepatoxicity; oxidative stress; reactive oxygen species; metabolic disorders; glycogen depletion; anaerobic glycolysis.
The rectal insufflation of a judicious dose of ozone, selected from that used in clinical practice, is able to promote oxidative preconditioning of oxidative stress tolerance preventing the hepatocellular damage mediated by free radicals. In order to evaluate the effects of ozone oxidative preconditioning on carbon tetrachloride-mediated hepatoxicity, the following experimental protocol was designed: group 1 (negative control, sunflower oil i.p.); group 2 (CCl4 in sunflower oil, 1 ml kg-1 i.p.); group 3 (15 ozone – oxygen pretreatments at a dose of 1 mg kg-1 via rectal insufflation + CCl4 as in group 2); group 4(ozone control group, 15 ozone-oxygen pretreatments + sunflower oil i.p.). Ozone pretreatment prevented glycogen depletion (as demonstrated by biochemical and histophatiological findings) and avoided lactate lipid peroxidation (as measured by thiobarbituric acid-reactive substances) and uric acid levels and inhibited superoxide dismutase activity.
All these deleterious effects induced by CCl4 were prevented by ozone pretreatment. The administration of ozone without CCl4 (ozone control group) did not produce any changes in the evaluated parameters. Our results showed that ozone treatment, in our experimental conditions, was able to prevent anaerobic glycolysis and oxidative stress induced by CCl4. Copyright © 2001 John Wiley & Sons, Ltd.