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Genotoxic effect of ozone in human peripheral blood leukocytes

Título
Genotoxic effect of ozone in human peripheral blood leukocytes
Autores

Silvia Díaz-Llera a,∗, Yanela González-Hernández b,
E.A. Prieto-González b, Angel Azoy a

a Centro de Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos,
Universidad de La Habana, Apdo. 14024, C.P. 11400, Havana, Cuba
b Centro de Investigaciones Biomédicas, Facultad de Medicina “Victoria de Girón”, Havana, Cuba

Fecha de Recibido

1999-05-20

Fecha de Publicación

2002-01-23

Ozone; Human leukocytes; DNA damage; SCGE; Comet assay; Catalase

Abstract

The genotoxic effect of ozone was studied in human leukocytes in vitro, using the single cell gel electrophoresis (SCGE) assay. Cell treatment for 1 h at 37 ◦Cwith 0.9-5.3mMO3 resulted in a dose-dependent increase ofDNAdamage, comparable to that induced by 4-40mM of H2O2, used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45-90 min at 37 ◦C, and prevented by pre-incubation of the cells with catalase (20ug/ml). These results demonstrate that O3 induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H2O2.

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