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A physicochemical investigation on the effects of ozone on blood

A physicochemical investigation on the effects of ozone on blood

Valter Travagli a, Iacopo Zanardi a, Antonella Silvietti b, Velio Bocci c,∗

a Department of Pharmaceutical Chemistry and Technology, University of Siena, Siena, Italy
b Central Laboratory of Clinical Analysis, Polyclinic, University of Siena, Siena, Italy
c Department of Physiology, University of Siena, Siena, Italy

Revista o sitio electrónico (URL) donde se publicó

International Journal of Biological Macromolecules

Fecha de Recibido


Fecha de Publicación


Erythrocytes; Ozone; Haemolysis; Viscosity; Capillary electrophoresis; Oxidative stress


Ozonation of either human whole blood or saline-washed erythrocytes causes considerable damage to the latter and this result has opened a controversy.With the benefit of hindsight, it appears logical that once erythrocytes are deprived of the potent antioxidants of plasma, they become very sensitive to the oxidant effects of ozone. The aim of the presentworkwas to perform a physical-chemical evaluation of some critical parameters able to clarify this issue.We have ascertained that when whole blood is exposed to the appropriate ozone doses used in human therapy, no damage ensues while saline-washed erythrocytes undergo conspicuous haemolysis. The dogma that ozone is always toxic is incorrect because its reactivity below the concentration of 80 ug/mL can be controlled by the plasmatic antioxidant system.


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