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Effects of Ozone Oxidative Preconditioning on TNF-x Release and Antioxidant-Prooxidant Intracellular Balance inMice During Endotoxic Shock

Título
Effects of Ozone Oxidative Preconditioning on TNF-x Release and Antioxidant-Prooxidant Intracellular Balance inMice During Endotoxic Shock
Autores

Zullyt B. Zamora,1 Aluet Borrego,1 Orlay Y. López,2 René Delgado,2 Ricardo González,1
Silvia Menéndez,1 Frank Hernández,1 and Siegfried Schulz3

1Department of Biomedicine, Ozone Research Center, National Center for Scientific Research, PO Box 6414, Havana, Cuba
2Department of Biomedicine, Center for Pharmaceutical Chemistry, PO Box 16042, Havana, Cuba
3Veterinary Services and Laboratory Animal Medicine, Philipps-University of Marburg, 35033 Marburg, Germany

Revista o sitio electrónico (URL) donde se publicó

RESEARCH COMMUNICATION

Fecha de Recibido

2003-09-25

Fecha de Publicación

2004-11-04

Abstract

Ozone oxidative preconditioning is a prophylactic approach, which favors the antioxidant-prooxidant balance for preservation of cell redox state by the increase of antioxidant endogenous systems in both in vivo and in vitro experimental models. Our aim is to
analyze the effect of ozone oxidative preconditioning on serum TNF- levels and as a modulator of oxidative stress on hepatic tissue in endotoxic shock model (mice treated with lipopolysaccharide (LPS)). Ozone/oxygen gaseous mixture which was administered intraperitoneally (0.2, 0.4, and 1.2mg/kg) once daily for five days before LPS (0.1mg/kg, intraperitoneal). TNF- was measured by cytotoxicity on L-929 cells. Biochemical parameters such as thiobarbituric acid reactive substances (TBARS), enzymatic activity of catalase, glutathione peroxidase, and glutathione-S transferase were measured in hepatic tissue. One hour after LPS injection there was a significant increase in TNF- levels in mouse serum. Ozone/oxygen gaseous mixture reduced serum TNF- levels in a dose-dependent manner. Statistically significant decreases in TNF- levels after LPS injection were observed in mice pretreated with ozone intraperitoneal applications at 0.2 (78%), 0.4 (98%), and 1.2 (99%). Also a significant increase in TBARS content was observed in the hepatic tissue of LPS-treatedmice, whereas enzymatic activity of glutathion-S transferase and glutathione peroxidase was decreased. However in ozone-treated animals a significant decrease in TBARS content was appreciated as well as an increase in the activity of antioxidant enzymes. These results indicate that ozone oxidative preconditioning exerts inhibitory effects on TNF- production and on the other hand it exerts influence on the antioxidant-prooxidant balance for preservation of cell redox state by the increase of endogenous antioxidant systems.

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